A new multiphoton microscopy module enables millisecond neural event imaging at single-neuron resolution.
At the Neuroscience 2024 conference (www.sfn.org), Bruker Corporation (Nasdaq: BRKR) today announced the release of the OptoVolt™ module, which further expands the advanced neuroscience research capabilities of Bruker’s Ultima multiphoton microscopy platform. OptoVolt accelerates imaging speeds to over 1000 frames per second to capture the millisecond dynamics of cell-to-cell neural communication. With the advent of fluorescent voltage indicators, researchers are excited to measure neural activity with higher temporal resolution than traditional fluorescent calcium indicators.
The OptoVolt module uniquely addresses the challenges of voltage imaging by providing a scanner technology that can operate at ultra-high speeds and with the signal-to-noise ratio required to detect individual neural events. Additionally, when combined with Bruker’s NeuraLight 3D Ultra® spatial light modulator (SLM) for 3D optogenetic activation, the entire platform allows researchers to investigate input-output relationships in neural ensembles with unprecedented temporal and spatial resolution. will be able to investigate.
OptoVolt allows extraction of fluorescent voltage traces. Image credit: Bruker
“We believe that voltage imaging will become an important tool in the study of neural circuits and systems neuroscience in the near future,” said Thomas Clandinin, a professor in the Department of Neurobiology at Stanford University. “We are excited about our new instrumentation, which expands the possibility of optically interrogating cells and intracellular signaling with the temporal resolution of individual action potentials.”
“We are proud to introduce this significant advance in neural circuit imaging capabilities due to the combination of unparalleled imaging speed and signal-to-noise performance that enables millisecond neural event detection with single-neuron resolution. ” added Dr. Xiaomei Li of Vice. President and General Manager of Bruker’s fluorescence microscopy business. “OptoVolt demonstrates how we pay attention to the evolving research needs of our customers and work closely with them to drive innovation. This direct collaboration allows us to We are building the versatile Ultima 2Pplus platform as the ideal solution for all-optical neuroscience research.”
About the OptoVolt module
The OptoVolt module integrates seamlessly with the Ultima 2Pplus multiphoton microscope, delivering kilohertz-level imaging speeds and capturing dynamics at up to 1000 frames/second. OptoVolt technology leverages novel voltage indicator proteins such as Stanford University’s ASAP4 and Baylor University’s JEDI-2P, ensuring compatibility with the latest developments in the field. The modular design of this system allows flexible adjustment between speed and signal strength while maintaining the depth and resolution of traditional two-photon imaging. Furthermore, the integration of OptoVolt and NeuraLight 3D Ultra SLM allows simultaneous optogenetic manipulation and voltage imaging, facilitating comprehensive functional mapping of neural circuits. This capability allows researchers to visualize action potentials and subthreshold membrane potentials across large neural networks, bridging the gap between single-cell electrophysiology and wide-field calcium imaging.
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